pH-Dependent Drug Absorption and Distribution

This interactive module demonstrates gastroenteric absorption and distribution of drugs as a function of pH in different body compartments, focusing on the relationship between logP, pKa, and pH-dependent distribution coefficient (logD).

Drug Properties

Weak Base

2.0

9.5

75

pH Environments

1.5

7.4

7.0

7.2

6.0

Calculations

logD Values

Stomach: -

Enteric (Small Intestine): -

Blood: -

Tissue: -

Urine: -

Ionization (%)

Stomach: -%

Enteric (Small Intestine): -%

Blood: -%

Tissue: -%

Urine: -%

Permeability

Gastric Absorption: -

Intestinal Absorption: -

Blood-Brain Barrier: -

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Key Concepts in pH-Dependent Drug Distribution

logP (Partition Coefficient): The logarithm of the octanol-water partition coefficient for the unionized form of a drug. This represents the intrinsic lipophilicity of the molecule and determines its ability to cross lipid membranes.

pKa (Acid Dissociation Constant): The pH at which 50% of the drug exists in ionized form and 50% in unionized form. For weak acids, lower pKa means stronger acid; for weak bases, higher pKa means stronger base.

logD (Distribution Coefficient): The pH-dependent distribution coefficient that accounts for both ionized and unionized forms of the drug at a specific pH. This is what actually determines drug behavior in biological systems.

Henderson-Hasselbalch Equations

For weak acids: - logD = logP + log(1/(1 + 10^(pH - pKa))) - % Ionized = 100 × 10^(pH - pKa) / (1 + 10^(pH - pKa))

For weak bases: - logD = logP + log(1/(1 + 10^(pKa - pH)))
- % Ionized = 100 × 10^(pKa - pH) / (1 + 10^(pKa - pH))

Clinical Implications

Gastric Absorption: Only unionized (lipophilic) forms can cross gastric mucosa. Weak acids are better absorbed in the acidic stomach, while weak bases have poor gastric absorption.

Intestinal Absorption: The slightly alkaline intestinal environment (pH ~6.5-7.5) favors absorption of weak bases over weak acids.

Blood-Brain Barrier: Only highly lipophilic, unionized molecules readily cross. The blood pH of 7.4 significantly affects brain penetration.

Renal Elimination: pH-dependent ionization affects both glomerular filtration and tubular reabsorption, allowing for pH manipulation in poisoning cases.

Physiological pH Values

  • Stomach: 1.5-3.5 (fasting), 3.0-5.0 (fed)
  • Small intestine: 6.5-7.5
  • Blood: 7.35-7.45
  • Intracellular: 6.8-7.2
  • Urine: 4.5-8.5 (variable)

Test your understanding of pH-dependent drug distribution:

1. Calculate logD for the current drug in blood (pH 7.4):

2. What percentage is ionized in the stomach?

3. Which compartment has the highest logD for this drug?