Endocarditis

Offending agent: Vancomycin-resistant Enterococcus faecium (VRE)

Drug of Choice: Daptomycin

Physicochemical Properties: - pKa: 2.8 (acidic drug; predominantly ionized at physiological pH) - logP: -0.1 (moderately hydrophilic with some lipophilic character)

Tissue Distribution Rationale: Daptomycin’s moderate logP (-0.1) and acidic pKa (2.8) provide balanced hydrophilic/lipophilic properties enabling good tissue penetration. Its calcium-dependent mechanism requires adequate tissue calcium concentrations, and its moderate lipophilicity allows penetration into cardiac tissues affected in endocarditis.

PK/PD Target: AUC/MIC ≥ 666, Cmin ≥ 24.3 mg/L (high dose for endocarditis)

Key PK Parameters for Endocarditis: - Volume of Distribution: 0.1 L/kg (poor tissue penetration) - Protein Binding: ~8% (high free fraction = 0.92) - Cardiac Tissue: Limited penetration, requires high systemic exposure - Dosing: 8-10 mg/kg/day for complex endocarditis

This interactive module demonstrates multiple dosing pharmacokinetics using patient-specific parameters.

Patient Generator

  • Age: - years
  • Sex: -
  • Height: - cm
  • Weight: - kg
  • Creatinine: - mg/dL

Fast (IV Bolus)

PK Parameters

1.0

0.08

0.1 L/kg

0.5

Dosing

700 mg

700 mg

24 hrs

10 days

Loading interactive chart…

20 mg/L

5 mg/L

Subject Drug Name Age Sex Ht (cm) Wt (kg) BMI IBW (kg) Adj IBW (kg) Creatinine (mg/dL) CrCl (mL/min) Loading Dose (mg) Dose (mg) Interval (hrs) Duration (days) Clearance (L/h) Volume (L) Fraction Unbound Vss,u (L) Bioavailability Half-life (h) Css,avg AUC0-24h Cmax AUC/Efficacy Cmax/Efficacy Time>Efficacy (%)

Endocarditis-Specific Pharmacokinetic Concepts

Cardiac Tissue Penetration Challenges: Endocarditis involves infected cardiac valves with poor vascularization and biofilm formation. Daptomycin has limited tissue penetration (Vd = 0.1 L/kg) but high systemic exposure compensates.

High-Dose Strategy for VRE Endocarditis: Target AUC₀₋₂₄/MIC ≥ 666 and Cmin ≥ 24.3 mg/L requires aggressive dosing (8-10 mg/kg/day). Higher doses may be needed for complex cases (up to 12 mg/kg/day).

Calcium-Dependent Activity: Daptomycin requires physiologic calcium concentrations for antimicrobial activity. Serum calcium should be monitored and maintained >8.5 mg/dL.

Protein Binding Considerations: Low protein binding (~8%) means 92% is active, contributing to excellent antimicrobial effect despite poor tissue penetration.

Unique Monitoring Requirements: - CPK levels: Monitor for myopathy (dose-related toxicity) - Pulmonary function: Rare but serious eosinophilic pneumonia - Renal function: Potential nephrotoxicity with high doses

Extended Treatment Duration: Endocarditis requires 4-6 weeks of therapy. Some cases may need surgical intervention combined with antimicrobial therapy for optimal outcomes.

Blood Sampling Strategy

In this simulation, blood samples are collected every 1 hour to provide a detailed view of how pharmacokinetic data is collected in clinical practice. This sampling frequency allows us to:

  • Capture both peak and trough concentrations
  • Observe the full absorption and elimination phases
  • Reduce the burden on patients compared to more frequent sampling
  • Provide sufficient data points for pharmacokinetic analysis

Simplified Pharmacokinetic Model

This simulation uses a simplified one-compartment model with first-order absorption that focuses on the minimum essential parameters needed to generate a multiple dosing pharmacokinetic plot:

Essential PK Parameters: - Clearance (CL): Automatically calculated from patient’s creatinine clearance using Cockcroft-Gault equation - Volume of Distribution (Vd): Drug-specific volume per kg multiplied by patient weight - Bioavailability (F): Fraction of dose reaching systemic circulation

Patient-Specific Factors: - Creatinine Clearance: Calculated using Cockcroft-Gault: CrCl = [(140 - age) × weight × (0.85 if female)] / (72 × serum creatinine) - Weight-Based Dosing: Volume of distribution scales directly with patient weight

Dosing Parameters: - Dose: Amount of drug administered per dose - Dosing Interval: Time between doses - Treatment Duration: Total length of treatment

This approach incorporates key patient-specific factors (age, sex, weight, creatinine) while maintaining the core functionality needed to understand multiple dosing pharmacokinetics and the clinical application of the Cockcroft-Gault equation.

Test your understanding by calculating the following parameters for the current patient:

1. Creatinine Clearance (mL/min):

2. Adjusted Body Weight (kg):

3. Volume of Distribution of Unbound Drug (L):

4. Time to Steady State - tss (hours):

5. Average Steady-State Concentration - Css,avg (mg/L):

6. Daptomycin PK/PD: Based on daptomycin’s pKa (2.8) and physiological pH (7.4), what percentage of the drug is ionized?

Hint: Use Henderson-Hasselbalch equation. For acids: ionized % = 100 × 10^(pH-pKa)/(1 + 10^(pH-pKa))

7. Cardiac Penetration: Why does daptomycin require high systemic exposure (8-10 mg/kg/day) for endocarditis treatment?

8. Mechanism Dependency: What unique feature of daptomycin’s mechanism affects its tissue distribution?